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fluo-8/am dye  (AAT Bioquest)

 
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    AAT Bioquest fluo-8/am dye
    Fluo 8/Am Dye, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluo-8%C2%AE%2C+am/fluo+8+am/10__1097_slash_aln__0000000000005630-89-26-29
    Average 90 stars, based on 1 article reviews
    fluo-8/am dye - by Bioz Stars, 2026-08
    90/100 stars

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    Image Search Results


    Yoda1 promotes YAP nuclear translocation through calcium ion influx and inhibits cell apoptosis. (a) Intracellular calcium ion (Ca 2+ ) influx levels in human gingival fibroblasts (HGFs) under various treatment conditions were detected using the Fluo-8 AM fluorescent probe. Representative fluorescence images (green) and quantitative analyses of fluorescence intensity are shown (n = 5 per group), with nuclei counterstained with DAPI (blue). (b) Immunofluorescence staining was used to detect the expression, distribution, and quantitative statistics of the YAP transcription factor (green) and cytoskeleton (F-actin, labeled with phalloidin) in HGF under different treatments (n = 3 per group), with nuclei counterstained with DAPI (blue). (c) Western blot analysis of apoptosis-related proteins Bax and Bcl-2 expression levels in HGFs under different treatments, with grayscale value quantification (n = 3 per group,all proteins levels are normalized to loading control, GAPDH). Quantitative data are presented as mean ± standard deviation (mean ± SD) from at least three independent experiments. Differences between groups were analyzed by one-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Yoda1-activated Piezo1 enhances mitophagy in human gingival fibroblasts to promote maxillofacial wound repair

    doi: 10.3389/fphar.2026.1803022

    Figure Lengend Snippet: Yoda1 promotes YAP nuclear translocation through calcium ion influx and inhibits cell apoptosis. (a) Intracellular calcium ion (Ca 2+ ) influx levels in human gingival fibroblasts (HGFs) under various treatment conditions were detected using the Fluo-8 AM fluorescent probe. Representative fluorescence images (green) and quantitative analyses of fluorescence intensity are shown (n = 5 per group), with nuclei counterstained with DAPI (blue). (b) Immunofluorescence staining was used to detect the expression, distribution, and quantitative statistics of the YAP transcription factor (green) and cytoskeleton (F-actin, labeled with phalloidin) in HGF under different treatments (n = 3 per group), with nuclei counterstained with DAPI (blue). (c) Western blot analysis of apoptosis-related proteins Bax and Bcl-2 expression levels in HGFs under different treatments, with grayscale value quantification (n = 3 per group,all proteins levels are normalized to loading control, GAPDH). Quantitative data are presented as mean ± standard deviation (mean ± SD) from at least three independent experiments. Differences between groups were analyzed by one-way ANOVA (* P < 0.05, ** P < 0.01, *** P < 0.001).

    Article Snippet: HGFs in the respective experimental groups were treated, and intracellular calcium ions were subsequently detected using Fluo-8 AM (Applygen).

    Techniques: Translocation Assay, Fluorescence, Immunofluorescence, Staining, Expressing, Labeling, Western Blot, Control, Standard Deviation